In Vitro Eye Irritancy Test of Polyoxyethylene Alkyl Derivatives Using a Reconstructed Rabbit Corneal Epithelium Model
نویسندگان
چکیده
منابع مشابه
In vitro eye irritancy test of polyoxyethylene alkyl derivatives using a reconstructed rabbit corneal epithelium model.
We have developed the Rabbit Corneal Epithelial (RCE) Model to evaluate the in vitro eye irritation potential of chemicals including pharmaceuticals, cosmetics and their raw ingredients. In the model, a stratified culture of rabbit corneal epithelial cells is grown at the air-liquid interface on an amnion acting as a parabasal membrane. The alkaline exposure was restored each day in the presenc...
متن کاملApplication of the reconstructed rabbit corneal epithelium model to assess the in-vitro eye irritant test of chemicals.
The rabbit corneal epithelium model (RCE model) was developed as a three-dimensional in vitro model to replace animal testing for the assessment of eye irritation. In the model, a stratified culture of rabbit corneal epithelial cells is grown at the air-liquid interface on collagen gel that acts as a parabasal membrane. Histological cross-sections show that the structure of the RCE model closel...
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The 7 amendment of the EU Cosmetic Directive will lead to the ban of animal testing for cosmetic ingredients in 2009 (acute tests). Many efforts have been made in order to find reliable and relevant alternatives methods to eye irritation testing. 3-D in vitro technology allows the evaluation of chemicals in conditions similar to in vivo use. In this study, we have used the SkinEthic Human Corne...
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Household products, shampoos, pesticides, weed killers, even riot control gases, are just some of the products tested for irritancy in the eyes of conscious rabbits. The Draize test, introduced in 1944, is cruelly simple. The substance under test is sprayed or instilled into one eye of an albino rabbit. Generally no pain relief is given and the test often proceeds for 7 days during which the co...
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Recently, safety evaluation tests that do not involve animal experiments have been prosperously developing. However, the optimal evaluation materials and methods for assessing ocular irritancy have not been well investigated. In this study, we determined the optimal evaluation method for testing ocular irritation using a human cultured corneal epithelium model (corneal model). In order to asses...
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ژورنال
عنوان ژورنال: Biological and Pharmaceutical Bulletin
سال: 2009
ISSN: 0918-6158,1347-5215
DOI: 10.1248/bpb.32.807